FAQ: My fusion protein is insoluble; is there anything I can do to get it expressed as soluble protein?
Reviews on methods to make correctly-folded protein in E. coli:
Georgiou, G. et al. (1996) Current Opinion in Biotechnology 7:190–197. Sahdev S. et al. (2008) Mol. Cell Biochem 307:249-64
Reviews on refolding:
Qoronfleh MW, et al. (2007) Protein Expr Purif 55(2):209-24.
Jungbauer A and Kaar W. (2007) J Biotechnol 128(3):587-96
Jungbauer A, et al. (2004) Curr Opin Biotechnol. 15(5):487-94
Vallejo, LF and Rinas,U (2004) Microb Cell Fact 3(1):11-22