HomeFAQsDo I really need to vortex the fragmentation reactions, as described in the protocol? Will pipetting up and down work just as well?
FAQ: Do I really need to vortex the fragmentation reactions, as described in the protocol? Will pipetting up and down work just as well?
For optimal results, it is very important to vortex the reactions exactly as described in the protocol. Pipetting up and down instead of vortexing results in sub-optimal fragmentation and variable results.
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