FAQ: Can the histones be used as substrates for Histone modification enzymes? Which ones?
The histone modification enzymes that we have tested are:
Histone H1°: CDK2-cyclin B (NEB #P6020)
Histone H3.1, H3.2 and H3.3: G9A Methyltransferase (NEB #M0235) and SET7 Methyltransferase (NEB #M0233)
Histone H4: PRMT1 Methyltransferase (NEB #M0221) and SET8 Methyltransferase (NEB #M0248)