Yes. The adaptor must be a T overhang adaptor and the final library yield may be slightly lower than when using the NEBNext Adaptors for Illumina®.
Guidelines for use (these apply for non-NEBNext, full length barcoded adaptors and P5 and P7 universal PCR primers.):
- Use 2.5 µl adaptor. Adaptors are typically used at 15-25 µM concentration. The optimal adaptor concentration and dilution would have to be determined experimentally.
- Skip the USER addition and the 15 min USER incubation. Add 3 µl water or TE to sample to make up volume difference for the next step.
- Sample bead cleanup conditions may have to be modified for other vendor oligos.
- PCR with NEBNext MSTC™ FFPE Master Mix: use PCR Primers supplied with the vendor adaptors used. Do NOT use NEBNext Index Oligo PCR primers.