HomeFAQsCan N-Glycopeptide Binding Protein bind an intact glycoprotein?
FAQ: Can N-Glycopeptide Binding Protein bind an intact glycoprotein?
Yes, N-Glycopeptide Binding Protein can bind intact glycoproteins. However, the binding between the N-Glycopeptide Binding Protein and an intact glycoprotein is impacted by the higher order structure of the protein, the locality, and the structure of the glycans. As a result, although it is possible to enrich intact glycoproteins using this binding protein (in an immobilized format but not in a filtration-based workflow), the effectiveness of pull-down will be glycoprotein dependent.
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