Glycerol-free and Lyo-compatible Reagents for Molecular Biology Workflows
NEB has been developing innovative enzymes used in molecular biology methods for 50 years. To meet the performance demands of today’s assays, which include functional, performance, and environmental requirements, scientists rely on accessing the most innovative and specialized enzyme formulations. NEB’s glycerol-free and lyo-compatible enzyme formulations meet the demands needed by scientists. Glycerol-free and lyo-compatible formulations provide scientists with the flexibility for:
- Ambient shipment and storage through lyophilization
- Automation and HPLC compatibility
- Improved fluid dynamic properties
NEB provides select glycerol-free enzymes through our catalog. Additionally, we have a variety of glycerol-free and lyo-compatible enzyme formulations not currently offered through our catalog, but which can be accessed through our OEM & Customized Solutions team. These non-catalog formulations can be filled to meet your concentration, volume and format needs. Contact the OEM & Customized Solutions Team to learn more.
Glycerol-free Products for Molecular Biology Workflows
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Application |
Glycerol-free Product Name* |
Product Description |
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Reverse transcription |
Unique in silico designed RNA-directed DNA polymerase coupled with a reversibly-bound aptamer that inhibits RTx activity below 40°C. |
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Isothermal amplification
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In silico designed homolog of Bacillus stearothermophilus DNA Polymerase I, Large Fragment (Bst DNA Polymerase, Large Fragment) with a reversibly-bound aptamer, which inhibits polymerase activity at temperatures below 45°C. |
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Bst 2.0 DNA Polymerase (Glycerol-free) |
Contains 5´→ 3´ DNA Polymerase activity and strong strand displacement activity but lacks 5´ → 3´ exonuclease activity. |
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Bst 3.0 DNA Polymerase (Glycerol-free) |
In silico designed homologue of Bacillus stearothermophilus DNA Polymerase I, Large Fragment engineered and fused to a novel nucleic acid binding domain for improved isothermal amplification performance and increased reverse transcription activity. |
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Bst DNA Polymerase, Large Fragment (Glycerol-free) |
The portion of the Bacillus stearothermophilus DNA Polymerase protein that contains the 5´ → 3´ polymerase activity, but lacks 5´ → 3´ exonuclease activity. |
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Bst DNA Polymerase, Full Length (Glycerol-free) |
Full-length polymerase from Bacillus stearothermophilus. It has 5´ → 3´ polymerase and double-strand specific 5´ → 3´ exonuclease activity but lacks 3´ → 5´ exonuclease activity. |
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PCR
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Q5® High Fidelity DNA Polymerase (Glycerol-free) |
High-fidelity, thermostable DNA polymerase with 3´ → 5´ exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification with an error rate ~280 fold lower than that of Taq DNA Polymerase. |
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Q5 Hot Start High-Fidelity DNA Polymerase (Glycerol-free) |
High-fidelity, thermostable, hot start DNA polymerase with 3´ → 5´ exonuclease activity, fused to a processivity enhancing Sso7d domain to support robust DNA amplification. The addition of an aptamer-based inhibitor allows room temperature reaction setup. |
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Hot Start Taq DNA Polymerase (Glycerol-free) |
Mixture of Taq DNA Polymerase and an aptamer-based inhibitor. |
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Taq DNA Polymerase (Glycerol-free) |
Thermostable DNA polymerase that possesses 5´ → 3´ polymerase activity and a 5´ flap endonuclease activity. |
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Hemo KlenTaq® (Glycerol-free) |
Truncated version of Taq DNA Polymerase, lacking the first 280 amino acids, and also contains mutations that make it resistant to inhibitors present in whole blood. |
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| 9°Nm™ DNA Polymerase (Glycerol-free) | 3´ → 5´ exonuclease-deficient version of the DNA polymerase from the hyperthermophilic marine archaea Thermococcus sp. 9°N. It is extremely thermostable and can be used for PCR as well as applications requiring strand displacement activity. | |
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Restriction endonuclease
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Nt.BstNBI (Glycerol-free) |
Site specific endonuclease that cleaves only one strand of DNA on a double-strand DNA substrate. The nicking endonuclease catalyzes a single strand break 4 bases beyond the 3´ side of the recognition sequence. |
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Exonuclease |
Lambda Exonuclease (Glycerol-free) |
DNA specific exonuclease that catalyzes the removal of nucleotides from linear or nicked double-stranded DNA in the 5´ to 3´ direction. |
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Phosphatase |
Apyrase (Glycerol-free) |
Highly active ATP-diphosphodyhydrolase that catalyzes the sequential hydrolysis of ATP to ADP and ADP to AMP releasing inorganic phosphate. |
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ssDNA Binding |
T4 Gene 32 Protein (Glycerol-free) |
Single-stranded DNA (ssDNA) binding protein required for bacteriophage T4 replication and repair. |
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Protease |
Thermolabile Proteinase K (Glycerol-free) |
Recombinant, heat-inactivated Proteinase K is engineered for complete inactivation at 55°C in 10 minutes with broad protease activity for digestion of proteins in nucleic acid preparations and other applications. |
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Proteome analysis
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Recombinant amidase, which cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides. |
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An amidase, which cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides. |
*If you require other enzymes in a glycerol-free format, please reach out to our OEM & Customized Solutions team to explore feasibility.
Contact the OEM & Customized Solutions Team to learn more.
Lyo-compatible Mixes
Lyo-compatible reagents from NEB are reagents and mixes whose formulations have been modified to remove components which inhibit lyophilization (examples: Glycerol, DMSO). Lyo-compatible reagents formulations do not contain excipients.
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Lyo-compatible Products |
Notes |
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|---|---|---|
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Isothermal amplification |
WarmStart Multi-Purpose LAMP/RT-LAMP 4X Master Mix (with UDG, Lyo-compatible) |
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Isothermal amplification |
Custom WarmStart LAMP 4X Master Mix (Lyo-compatible) |
LAMP/RT-LAMP Mix without UDG |
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RT-qPCR |
Luna® One-Step 5X Reaction Mix (Lyo-compatible) |
Core components of LyoPrime Luna Probe One-Step RT-qPCR Mix (with UDG) |
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Reverse Transcription |
Luna WarmStart RT 300X Enzyme Mix (with UDG, Lyo-compatible) |
Resource Links
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