Monarch® Spin Kits for DNA Cleanup and Gel Extraction
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Monarch kits are an excellent choice for cleaning up and concentrating DNA after enzymatic reactions and from agarose gels. Highly-pure DNA is ready in minutes in volumes as low as 5 µl. Monarch kits are designed with sustainability in mind and buffers and columns are available separately for added convenience.
Reasons to choose Monarch Spin Kits for DNA Cleanup
- Elute in as little as 6 µl and prevent buffer/salt carryover with the unique column design
- Purify fragments of various sizes efficiently with the Gel Extraction Kit
- Effectively remove enzymatic reaction components including primers
- Purify oligos and small DNA fragments with the PCR & DNA cleanup kit using a simple protocol modification
- Fast and simple product workflows (cleanup DNA from enzymatic reactions in just 5 minutes!)
Unique Column Design
Not only are Monarch columns designed to use less plastic, but they are also optimized to eliminate buffer retention; Monarch columns are designed without a retaining ring, thus preventing salts from carrying over into your eluted samples. The tapered design of the column also enables low volume elution (≥ 5 µl).
Monarch DNA Cleanup Column Design
Efficient DNA Purification from Gels and Enzymatic Reactions
Monarch Spin kits enable efficient purification of highly-pure and highly concentrated DNA from enzymatic reactions and agarose gels. Purified DNA is ready for use in downstream manipulations such as ligations and restriction digests. Together with our enhanced buffer system, Monarch columns deliver excellent performance.
Monarch Spin DNA Gel Extraction Kit
For the extraction, purification, and concentration of up to 5 µg of high-quality, double-stranded DNA from agarose gels. This new kit is effective for a wide range of DNA sizes and features a rapid 10 min protocol and the ability to elute in as little as 5 µl. It recovers DNA better than the leading supplier and is more highly concentrated for greater downstream utility!
Monarch Spin PCR & DNA Cleanup Kit
This new kit provides a rapid method for the purification and concentration of up to 5 ug of high-quality, double-stranded and single-stranded DNA from enzymatic reactions such as PCR, restriction digestion, and reverse transcription. Purify small DNA fragments, including oligonucleotides, with this single kit, elute in as little as 5 µl, and complete the entire workflow in only 5 minutes. The kit produces more concentrated DNA with better DNA recovery and purity compared to the leading supplier!
Purify oligos and small DNA fragments
The Monarch Spin PCR & DNA Cleanup Kit protocol can be modified to enable the purification of ssDNA, oligonucleotides, and other small DNA fragments. The Oligonucleotide Cleanup protocol efficiently removes unincorporated nucleotides, short oligos, dyes, enzymes, and salts from labeling and other enzymatic reactions. The modified protocol utilizes the same columns and bind/wash/elute workflow of the Monarch Spin PCR & DNA Cleanup Kit with > 70% recovery and cleanup of oligonucleotides ≥ 15 bp (dsDNA) or ≥18 nt (ssDNA).
Available Products:
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Monarch Spin PCR and DNA Cleanup Kit (5 ug)
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Monarch DNA Cleanup Columns (5 μg) |
I often purify small amounts of inserts for ligations, so I appreciate the ability to elute in small volumes. I also very much appreciate the reduction of paper and plastic waste.
