Monarch^® RNA Cleanup Columns (500 μg)

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
T2057L     25       Monarch® RNA Cleanup Columns (500 μg) T2057-1 25 2 x 50 columns Not Applicable   Monarch® Spin Collection Tubes T2118-1 25 2 x 50 tubes Not Applicable

1. Separation of Large and Small RNA into Fractions using the Monarch^® RNA Cleanup Kits

• Avoiding Ribonuclease Contamination
• Guidelines for RNA Quantitation
• Guidelines for Working with RNA During RNA Cleanup

• A Practical Guide to Analyzing Nucleic Acid Concentration and Purity with Microvolume Spectrophotometers

1. What is the maximum binding capacity of the Monarch RNA Cleanup Column provided with the Monarch RNA Cleanup Kit?
2. What is the smallest volume of nuclease-free water that can be used for elution with the Monarch RNA Cleanup Columns?
3. Can I get better recovery with the Monarch RNA Cleanup Kits if I do a second elution with my eluent from the first elution?
4. What factors affect my (A260/A230) when using the Monarch RNA Cleanup Kits?
5. What size RNA can be purified with the Monarch RNA Cleanup Kit?
6. Can I use the Monarch RNA Cleanup Kit to cleanup up my DNase I-treated RNA?
7. Can I use the Monarch RNA Cleanup Kits to purify RNA from agarose gels?
8. Can I use the Monarch RNA Cleanup Kits to cleanup RNA after a TRIzol®/chloroform extraction?
9. Can I use the Monarch RNA Cleanup Kits to cleanup my in vitro transcription (IVT) reaction?
10. Are the Monarch RNA Cleanup Kits (NEB # T2030, #T2040, #T2050) compatible with Luna RT-qPCR reagents?
11. Are the Monarch RNA Cleanup Kits (NEB #T2030, #T2040, #T2050) compatible with NEBNext reagents for RNA library prep?
12. My sample turned cloudy after adding the Monarch RNA Cleanup Binding Buffer and ethanol. Is this normal?
13. Why do I need to incubate my column for 5 minutes with elution buffer (nuclease-free water)?
14. How can I assess RNA integrity and purity?
15. Are the columns in the Monarch RNA Cleanup Kits the same as those in the Monarch Total RNA Miniprep Kit (NEB #T2010)?
16. Can I purchase Monarch buffers and columns separately?
17. Can I do an on-column DNase I treatment with the Monarch RNA Cleanup Columns?
18. Can the Monarch RNA Cleanup Kits (NEB #T2030, #T2040, #T2050) also be used to purify DNA?
19. I would like to clean up  ~150 µg of RNA. Would it be better to use multiple 50 µg columns (NEB #T2040/#T2047) or a single 500 µg column (NEB #T2050/#T2057)?
20. I notice that I'm applying force to fit the column into a collection tube. Should I be doing this? What collection tubes are compatible with columns?

• Troubleshooting Guide for RNA Cleanup

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• T2057L_v2
• T2057L_v3

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• T2057L_v1_0011806
• T2057L_v1_10023558
• T2057L_v1_10041697
• T2057L_v1_10059921
• T2057L_v1_10068608
• T2057L_v2_10099690
• T2057L_v2_10126365
• T2057L_v3_10163958
• T2057L_v3_10197192
• T2057L_v3_10237540

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.