Thermolabile USER® II Enzyme Standard Protocol (M5508)
Thermolabile USER® II Enzyme |
1 unit (1 µl) |
Uracil-containing DNA |
up to 10 pmol |
rCutSmart™ Buffer (10X) |
5 µl (1X) |
Total Reaction Volume |
50 µl |
Incubation Temperature |
37°C |
Incubation Time |
15 min. |
Heat Inactivate for 10 min at 65°C |