Protocol for RecJf (NEB #M0264)
RecJf is a single-stranded DNA specific exonuclease that catalyzes the removal of deoxynucleotide monophosphates from DNA in the 5´ → 3´ direction.
- Set-up a typical reaction as follows:
COMPONENTS | 50 μl REACTION |
---|---|
DNA | Up to 1 µg |
NEBuffer 2 (10X) | 5 µl (1X) |
RecJf | 1 µl (30 units) |
Nuclease-free H2O (NEB #B1500S) | up to 50 µl |
- Incubate at 37°C for 20 minutes.