Where can I find protocols for using NEBNext® DNA Library Prep reagents?

Use the table below to find the appropriate your library prep, based on sample type and kit being used.

NEBNext Adaptor (Non-Indexed):

  Ultra II DNA Ultra II FS DNA
Input Amount Input Amount
Full Range ≤ 100 ng ≥ 100 ng > 550 bp Full Range
Find protocols for use with:
NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®
(NEB #E7645, #E7103S/L) 		NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®        
NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina®
(NEB #E7805S/L, #E6177S/L) 		  Inputs ≤ 100 ng

Inputs ≥ 100 ng

Large Fragment sizes (> 550 bp)

  
NEBNext® Ultra™ II Ligation Module
(NEB #E7595S/L)		 NEBNext Ultra II End Repair/dA-Tailing Module       NEBNext Ultra II FS DNA Module

 

Unique Dual Index UMI Adaptor:

  Ultra II DNA Ultra II FS DNA
PCR PCR Free PCR PCR Free
Input Amount Input Amount
Full Range
 Full Range ≤ 100 ng ≥ 100 ng > 550 bp 100 ng to 500 ng 50 ng to 99 ng
Find protocols for use with:
NEBNext® Ultra™ II DNA PCR-free Library Prep Kit for Illumina®
(NEB #E7410S/L, #E7415S/L) 		  NEBNext® Ultra™ II DNA PCR-free Library Prep Kit for Illumina®             
NEBNext® Ultra™ II FS DNA PCR-free Library Prep Kit for Illumina®
(NEB #E7430S/L, #E7435S/L)		             High inputs (100 ng to 500 ng) Low inputs (50 ng to 99 ng)
NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina® for use with NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors DNA)
(NEB #E7805S/L, #E6177S/L)		       Inputs ≤ 100 ng

Inputs ≥ 100 ng

Large Fragment sizes (> 550 bp)

    
NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® for use with NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors DNA)
(NEB #E7645, #E7103S/L) 		NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® for use with NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors DNA)              
NEBNext® Ultra™ II Ligation Module for use with NEBNext Multiplex Oligos for Illumina® (Unique Dual Index UMI Adaptors DNA)
(NEB #E7595S/L) 		NEBNext Ultra II End Repair/dA-Tailing Module   NEBNext Ultra II FS DNA Module          

* Please also see manual for NEBNext® Multiplex Oligos for Illumina® Manual for Kit Sets 1-4: NEBNext® Multiplex Oligos for Illumina® (Unique Dual Index UMI Adaptors DNA Sets 1-4) NEB #E7395S/L, NEB #E7874S/L, NEB #E7876S/L, NEB #E7878S/L

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