Home Forms NEBuilder^® HiFi DNA Assembly Master Mix Sample Request Form Thank you

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NEBUILDER HIFI DNA ASSEMBLY^® Removal of 3´ end Mismatches

Did you know that one of the advantages of NEBuilder HiFi is that it removes 3’ and 5’ -end mismatch sequences prior to fragment assembly? Learn how!
Construction of an sgRNA-Cas9 Expression Vector via an ssOligo Bridge

Learn how you can use NEBuilder HiFi to generate an sgRNA-Cas9 expression vector with a single-stranded oligo bridge.
NEBUILDER^® HiFi: the next generation of DNA Assembly

In this webinar, we will explore some of the latest applications for NEBuilder HiFi DNA Assembly. View full Q & A summary.
Introduction to NEBUILDER HIFI DNA ASSEMBLY^®

Find out how NEBuilder® HiFi DNA Assembly can reliably join DNA fragments in a single tube, isothermal reaction, with advantages over NEB Gibson Assembly®.
Primer Design and Fragment Assembly Using NEBuilder HiFi DNA Assembly^® or Gibson Assembly^®

Watch an interactive tutorial on primer design to see how simple it really is to clone with either NEBuilder HiFi DNA Assembly or the Gibson Assembly Cloning Kit.
NEBUILDER^® HIFI DNA ASSEMBLY^® : Bridging dsDNA with a ssDNA Oligo

Learn how NEBuilder® HiFi DNA Assembly bridges dsDNA with a ssDNA oligo.