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DNA Ligases

NEB offers a variety of ligases for DNA research. Many of these enzymes are recombinant, and all offer the quality and value you have come to expect from our products.

The largest selection of DNA Ligases for your ligation needs


With over 45 years of experience in the development and production of enzymes for molecular biology, NEB offers the most extensive selection of high-quality and performance-optimized DNA ligases and master mixes to streamline your experiments.

A few of our more popular standalone ligases and ligase kits available are:

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DNA Ligases
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9°N™ DNA Ligase

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Blunt/TA Ligase Master Mix

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E. coli DNA Ligase

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ElectroLigase®

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HiFi Taq DNA Ligase

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Hi-T4™ DNA Ligase

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Immobilized T4 DNA Ligase

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Instant Sticky-end Ligase Master Mix

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NEBNext® Quick Ligation Module

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NEBridge® Ligase Master Mix

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Quick Ligation™ Kit

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Salt-T4® DNA Ligase

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SplintR® Ligase

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StickTogether™ DNA Ligase Buffer

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T3 DNA Ligase

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T4 DNA Ligase

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T4 DNA Ligase Reaction Buffer

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T7 DNA Ligase

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Taq DNA Ligase

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Taq DNA Ligase Reaction Buffer

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Thermostable 5´ App DNA/RNA Ligase

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Protocols for DNA Ligases
Application Notes for DNA Ligases
Reported Activities and Applications for T4 Ligases
Relative Efficiency of T4 Ligases on Nicked Substrates
Legal Information

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. All other trademarks are the property of their respective owners. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

 


Videos

  • Fidelity-and-Bias-ligation

    Ligase Fidelity and Bias in End-Joining Ligation: Enabling complex, multi-fragment Golden Gate DNA Assembly

    In this webinar, you will learn about our recently developed a single-molecule sequencing method to characterize end-joining ligase fidelity (discrimination against ligating mismatched overhangs) and bias (sequence preferences) for short cohesive ends in a high throughput manner. This method allows determination of the relative frequency of all ligation products with or without mismatches, the position-dependent frequency of each mismatch, and sequence-dependent biases in ligation efficiency. We have applied the fidelity and bias data to optimize design of Golden Gate-type assemblies, allowing selection of overhang sets with minimized mismatch potential and high efficiency. 

  • DNA Ligase

    DNA Ligase Fidelity: When does it matter?

    High fidelity polymerases are everywhere—but why would you need a high fidelity ligase? And what do we even mean by “fidelity” when we’re talking about ligation? In this webinar, NEB Scientist and ligase expert Greg Lohman discusses mismatch ligation by DNA ligases and the molecular diagnostics applications that depend on the use of high-fidelity DNA ligases like NEB’s HiFi Taq DNA Ligase to detect single base differences in DNA.

  • DNA Ligase

    DNA Ligation

    Ligation, the process of joining DNA fragments with a DNA ligase, proceeds in three steps. Learn more about the function of ligation with our quick tutorial animation.

  • DifficultLigation_thumb

    Are some ligations more difficult than others?

    Ligation of blunt ends and single-base overhangs require optimized reaction conditions.

  • WhyUsePEG_thumb

    Why do I need to add PEG to my DNA ligation?

    Polyethylene glycol (PEG) is an important reagent in ligation reactions, find out why.

  • BestReactionTimes_thumb

    What are the best conditions for DNA ligation?

    Find out how the downstream application dictates the best reaction conditions for ligation.

  • BestRatios_thumb

    What molar ratios should I use for DNA Ligation?

    The optimal reactant ratio is contingent upon the downstream application.

Learn More

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