HomeFAQsCan I completely substitute a modified NTP for the canonical NTP counterpart? Will complete substitution impact yield?
FAQ: Can I completely substitute a modified NTP for the canonical NTP counterpart? Will complete substitution impact yield?
Yes. Complete substitution of modified NTPs for the canonical NTP is possible. This may slightly reduce IVT yield compared to reactions using only canonical NTPs. Minimum yield for NEB RNA synthesis kits will be achieved with complete substitution of modified NTPs.
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