5-Methyl-Cytidine-5’-Triphosphate (5-Methyl-CTP)

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
N0432S     -20       5-Methyl-Cytidine-5’-Triphosphate (5-Methyl-CTP) N0432SVIAL -20 1 x 0.1 ml 100 mM

Companion Products

• N1-Methyl-Pseudouridine-5’-Triphosphate (N1-Methyl-Pseudo-UTP)
• Pseudouridine-5’-Triphosphate (Pseudo-UTP)
• 5-Methoxy-Uridine-5’-Triphosphate (5-Methoxy-UTP)

1. K. Karikó, M. Buckstein, H. Ni, D. Weissman (2005). Immunity. 23(2), 165-175, DOI: 10.1016/j.immuni.2005.06.008
2. O. Andries, S. McCafferty, S. C. De Smedt, R. Weiss, N. N. Sanders, T. Kitada (2015). J. Controlled Release. 217, 337. DOI: https://doi.org/10.1016/j.jconrel.2015.08.051,
3. B. Li, X. Luo, Y. Dong (2016). Bioconjugate Chem. 27, 3, 849-853, DOI: https://doi.org/10.1021/acs.bioconjchem.6b00090,

1. Can modified ribonucleotides be used with HiScribe^® T7 High Yield RNA Synthesis Kit (NEB #E2040)?
2. Can I completely substitute a modified NTP for the canonical NTP counterpart? Will complete substitution impact yield?
3. Can I use more than one modified NTP in the same IVT? Will this impact yield?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• N0432S_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• N0432S_v1_10256648

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• 5-Methyl-Cytidine-5’-Triphosphate (5-Methyl-CTP)

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.