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CLIP-tag™ Substrates

NEB offers a large selection of fluorescent labels (substrates) for CLIP-tag fusion proteins. CLIP-tag™ substrates consist of a fluorophore conjugated to a cytosine leaving group via a benzyl linker. Substrates label the CLIP-tag without the need for additional enzymes. Cell-permeable substrates (CLIP-Cell™) are suitable for both intracellular and cell-surface labeling, whereas non-cell-permeable substrates (CLIP-Surface™) are specific for fusion proteins expressed on the cell surface only.

NEB offers a large selection of fluorescent labels (substrates) for CLIP-tag fusion proteins. CLIP-tag™ substrates consist of a fluorophore conjugated to a cytosine leaving group via a benzyl linker. Substrates label the CLIP-tag without the need for additional enzymes. Cell-permeable substrates (CLIP-Cell™) are suitable for both intracellular and cell-surface labeling, whereas non-cell-permeable substrates (CLIP-Surface™) are specific for fusion proteins expressed on the cell surface only.

CLIP-tag™, CLIP-Cell™ and CLIP-Surface™ are trademarks of New England Biolabs, Inc.

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CLIP-tag™ Substrates
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CLIP-Biotin

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CLIP-Cell™ 505

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CLIP-Cell™ TMR-Star

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CLIP-Surface™ 488

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CLIP-Surface™ 547

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CLIP-Surface™ 647

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FAQs for CLIP-tag™ Substrates
Protocols for CLIP-tag™ Substrates
    Publications related to CLIP-tag™ Substrates
    • Schulz C. and Köhn M. (2008) Simultaneous protein tagging in two colors Chem Biol; 15, PubMedID: 18291310
    • Gautier A. et al. (2008) An engineered protein tag for multiprotein labeling in living cells Chem Biol; 15, 128-136. PubMedID: 18291317
    • Gautier A. et al. (2009) Selective cross-linking of interacting proteins using self-labeling tags J Am Chem Soc; 131, 17954-17962 . PubMedID: 19916541
    • Maffei, M., Morelli, C., Graham, E., Patriarca, S., Donzelli, L., Doleschall, B., de Castro, Reis, F., Nocchi, L., Chadick, C.H., Reymond, L., Correa, I.R., Jr., Johnsson, K., Hackett, J.A., Heppenstall, P.A (2019) A ligand based system for receptor specific delivery of proteins Sci Rep; 9(1), 19214.. PubMedID: 31844114, DOI: 10.1038/s41598-019-55797-1
SNAP-tag®, CLIP-tag™ and ACP/MCP-tag Substrate Selection Chart
NEB offers a large selection of fluorescent labels (substrates) for SNAP-, CLIP-, ACP- and MCP-tag fusion proteins.
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Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. All other trademarks are the property of their respective owners. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

 


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