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Competent Cells

Choose the right cells for your cloning and protein expression applications from NEB’s portfolio of high efficiency competent cell strains.

Cloning Strains

For cloning experiments choose from several high efficiency competent cell strains. These E. coli strains are T1 phage resistant and are endA deficient for high quality plasmid preparations. Additionally, all competent cells from NEB are free of animal products.

Yeast

NEB offers chemically competent Kluyveromyces lactis cells and variants of this strain that have been tailored for specific protein expression needs. These cells are suitable for transformation with any of our linearized pKLAC series expression vectors.

E. coli for Protein Expression

NEB offers several strains with varying levels of expression control, each with T1 phage resistance and extremely high transformation efficiencies.


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Competent Cells
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NEB Cloning Competent E. coli Sampler

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NEB Tube Opener

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NEBExpress® E. coli Lysis Reagent

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NEBExpress® T4 Lysozyme

Cloning Competent Cell Strains

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dam/dcm Competent E. coli

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NEB Cloning Competent E. coli Sampler

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NEB Tube Opener

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NEB® 10-beta Competent E. coli (High Efficiency)

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NEB® 10-beta Electrocompetent E. coli

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NEB® 5-alpha Competent E. coli (High Efficiency)

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NEB® 5-alpha Competent E. coli (Subcloning Efficiency)

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NEB® 5-alpha F'Iq Competent E. coli (High Efficiency)

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NEB® Stable Competent E. coli (High Efficiency)

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NEB® Turbo Competent E. coli (High Efficiency)

E. coli Expression Strains

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BL21 Competent E. coli

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BL21(DE3) Competent E. coli

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Lemo21(DE3) Competent E. coli

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NEB Tube Opener

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NEBExpress® Competent E. coli (High Efficiency)

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NEBExpress® Iq Competent E. coli (High Efficiency)

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NEBExpress® MBP Fusion and Purification System

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NiCo21(DE3) Competent E. coli

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SHuffle® Express Competent E. coli

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SHuffle® T7 Competent E. coli

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SHuffle® T7 Express Competent E. coli

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SHuffle® T7 Express lysY Competent E. coli

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T7 Express Competent E. coli (High Efficiency)

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T7 Express lysY Competent E. coli (High Efficiency)

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T7 Express lysY/Iq Competent E. coli (High Efficiency)

Yeast Expression Strains

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K. lactis GG799 Competent Cells

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NEB Tube Opener

Competent Cells Buffers & Diluents

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NEB® 10-beta/Stable Outgrowth Medium

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SOC Outgrowth Medium

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Yeast Carbon Base Medium Powder

Cloning Competent Cell Strains
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dam/dcm Competent E. coli

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NEB Cloning Competent E. coli Sampler

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NEB Tube Opener

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NEB® 10-beta Competent E. coli (High Efficiency)

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NEB® 10-beta Electrocompetent E. coli

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NEB® 5-alpha Competent E. coli (High Efficiency)

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NEB® 5-alpha Competent E. coli (Subcloning Efficiency)

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NEB® 5-alpha F'Iq Competent E. coli (High Efficiency)

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NEB® Stable Competent E. coli (High Efficiency)

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NEB® Turbo Competent E. coli (High Efficiency)

E. coli Expression Strains
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BL21 Competent E. coli

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BL21(DE3) Competent E. coli

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Lemo21(DE3) Competent E. coli

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NEB Tube Opener

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NEBExpress® Competent E. coli (High Efficiency)

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NEBExpress® Iq Competent E. coli (High Efficiency)

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NEBExpress® MBP Fusion and Purification System

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NiCo21(DE3) Competent E. coli

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SHuffle® Express Competent E. coli

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SHuffle® T7 Competent E. coli

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SHuffle® T7 Express Competent E. coli

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SHuffle® T7 Express lysY Competent E. coli

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T7 Express Competent E. coli (High Efficiency)

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T7 Express lysY Competent E. coli (High Efficiency)

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T7 Express lysY/Iq Competent E. coli (High Efficiency)

Yeast Expression Strains
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K. lactis GG799 Competent Cells

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NEB Tube Opener

Competent Cells Buffers & Diluents
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NEB® 10-beta/Stable Outgrowth Medium

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SOC Outgrowth Medium

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Yeast Carbon Base Medium Powder


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Competent Cells includes these subcategories:
Cloning Competent Cell Strains
E. coli Expression Strains Information
Yeast Expression Strains Information
FAQs for Competent Cells
Protocols for Competent Cells
Application Notes for Competent Cells
    Publications related to Competent Cells
    • Hibender, S. Landeta, C., Berkmen, M., Beckwith, J., Boyd, D. (2017) Aeropyrum pernix membrane protein VKOR promotes protein disulfide bond formation in two subcellular compartments. Microbiology; 163 (12), 1864-1869. PubMedID: 291309344​
    • Wagner, S., Klepsch, M., Schlegel, S., Appel, A., Draheim, R., Tarry, M., Hö, M., van Wijk, K.J., Slotboom, D.J., Persson, J.O. and de Gier, J. (2008) Tuning Escherichia coli for membrane protein overexpression Proc Natl Acad Sci U S A; 105 , 14371-14376 .
    • Ren, G., Ke, N. and Berkmen, M. (2016) Use of the Shuffle Strains in Production of Proteins. Curr Protoc Protein Sci; Aug 1, 1;85:5.26.1-5.26.21.. PubMedID: 27479507 , DOI: 10.1002/cpps.11.
    • Leith, E.M., O'Dell, W.B., Ke, N., McClung, C., Berkmen, M., Bergonzo, C., Brinson, R.G., Kelman, Z (2019) Characterization of the internal translation initiation region in monoclonal antibodies expressed in Escherichia coli J Biol Chem; 294(48), 18046-18056.. PubMedID: 31604819, DOI: 10.1074/jbc.RA119.011008
    • Reuter, W.H., Masuch, T., Ke, N., Lenon, M., Radzinski, M., Van Loi, V., Ren, G., Riggs, P., Antelmann, H., Reichmann, D., Leichert, L.I., Berkmen, M (2019) Utilizing redox-sensitive GFP fusions to detect in vivo redox changes in a genetically engineered prokaryote Redox Biol; 26, 101280. PubMedID: 31450103, DOI: 10.1016/j.redox.2019.101280
    • Reddy, P.T., Brinson, R.G., Hoopes, J.T., McClung, C., Ke, N., Kashi, L. (2018) Platform development for expression and purification of stable isotope labeled monoclonal antibodies in Escherichia coli. mAbs MAbs; 10 (7), 992-1002. PubMedID: 30060704, DOI: 10.1080/19420862.2018.1496879
    • Schlegel, S., Klepsch, M., Gialama, D., Wickström, D., Slotboom, D.J. and de Gier, J. (2010) Revolutionizing membrane protein overexpression in bacteria Microb Biotechnol; 3 , 403-411 .
    • Narayanan, A., Ridilla, M. and Yernool, D.A. (2010) Restrained expression, a method to overproduce toxic membrane proteins by exploiting operator–repressor interactions Protein Sci; 20 , 51-61 .
    • Agrawal, A., Bisharyan, Y., Papoyan, A, Bednenko, J., Cardarelli, J., Yao, M., Clark, T., Berkm​en, M., Ke, N., Colussi, P. (2019) Fusion to Tetrahymena thermophila granule lattice protein 1 confers solubility to sexual stage malaria antigens in Escherichia coli. Protein Expr Purif; 153, 7-17. PubMedID: 30081196, DOI: 10.1016/j.pep.2018.08.001.
NEB Competent E. coli Strain Selection
 
NEB offers several stains with varying levels of expression control, each with phage T1 resistance and extremely high transformation efficiencies.
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Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. All other trademarks are the property of their respective owners. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

 


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