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DNA Modifying Enzymes & Cloning Technologies

NEB offers a comprehensive list of tools and reagents for DNA manipulation. These include ligases, nucleases, methyltransferases, DNA repair proteins, recombinases and more.

NEBcloner is a guide for selecting appropriate products and viewing protocols for steps in the cloning workflow.

Advances in molecular biology require constant innovation and forward progress in the tools and techniques at the core of the field. New England Biolabs is committed to providing a wide variety of essential reagents that serve to advance the frontier of DNA-based manipulations.

NEB’s enzymology expertise sets it apart from competitors, allowing us to produce enzymes for molecular biology that deliver; our highly pure enzymes, over 250 of which are recombinant, offer exceptional performance and value.

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Argonautes

DNA Ligases

DNA Repair Enzymes and Structure-specific Endonucleases

Methyltransferases

Exonucleases and Non-specific Endonucleases

Phosphatases

Recombinases

ssDNA Binding Proteins

Kinases

DNA Labeling

Other

Argonautes

DNA Ligases

DNA Repair Enzymes and Structure-specific Endonucleases

Methyltransferases

Exonucleases and Non-specific Endonucleases

Phosphatases

Recombinases

ssDNA Binding Proteins

Kinases

DNA Labeling

Other

Choose Type:

DNA Modifying Enzymes & Cloning Technologies includes these subcategories:

Argonautes
DNA Ligase Products
DNA Repair Enzymes and Structure-specific Endonucleases
Methyltransferases
Exonucleases and Non-specific Endonucleases
Phosphatases
Recombinases
ssDNA Binding Proteins
Kinases
DNA Labeling
Other Products

Protocols for DNA Modifying Enzymes & Cloning Technologies

Application Notes for DNA Modifying Enzymes & Cloning Technologies

Joining of Difficult to Ligate dsDNA Fragments with Blunt TA Ligase Master Mix

Tools & Resources

Webinars

Quick Tips - Can I make multiple mutations with the Q5 site-directed mutagenesis kit?

Selection Tools

DNA Ligase Selection Chart

Properties of DNA and RNA Ligases

Troubleshooting Guides

Troubleshooting Guide for Cloning

Troubleshooting Tips for Ligation Reactions

Usage Guidelines

Tips for Maximizing Ligation Efficiencies

Traditional Cloning Quick Guide

Posters

miRNA Detection by Ligation and Amplification of Complementary DNA Oligos Using SPlintR® Ligase (2015)

A novel enzyme isolated from a hot spring metagenomic DNA library defines a new CAZy family (GH154) of exosialidases with an inverting catalytic mechanism (2018)

Single-Strand Specific, Plasmid Borne DNA Methyltransferases M.BceJIII and M.EcoGIX Regulate Plasmid and Single-Stranded Phage Replication. (2017)

Choosing the Right Exonuclease (2019)

Genome sequences of the human filarial parasites Mansonella perstans and Mansonella ozzardi

Genomes of the Wolbachia endosymbionts from the human filarial parasites Mansonella perstans and Mansonella ozzardi reveal multiple origins of nematode-Wolbachia symbiosis

Application of High Complexity Golden Gate Assembly to Rapid Engineering of Bacteriophage Genomes

Prediction of Golden Gate Assembly GGA Using a Comprehensive Analysis of T4 DNA Ligase End-Joining Fidelity and Bias (2018)

Publications related to DNA Modifying Enzymes & Cloning Technologies

Yao, K., Muruvanda, T., Roberts, R.J., Payne, J., Allard, M.W. and Hoffmann, M. (2016) Complete Genome and Methylome Sequences of Salmonella enterica subsp. enterica Serovar Panama (ATCC 7378) and Salmonella enterica subsp. enterica Serovar Sloterdijk (ATCC 15791). Genome Announc; Mar 17;4(2)., PubMedID: 26988049
Morgan, R.D., Luyten, Y.A., Johnson, S.A., Clough, E.M., Clark, T.A. and Roberts, R.J. (2016) Novel m4C modification in type I restriction-modification systems. Nucleic Acids Res; Nov, 2;44(19):9413-9425. PubMedID: 27580720
Anton, B.P., Harhay, G.P., Smith, T.P., Blom, J. and Roberts, R.J. (2016) Comparative Methylome Analysis of the Occasional Ruminant Respiratory Pathogen Bibersteinia trehalosi. PLoS One; Aug 24, 11(8):e0161499.. PubMedID: 27556252
Ee, R., Lim, Y.L., Yin, W.F., See-Too, W.S., Roberts, R.J. and Chan, K.G. (2016) Novel Methyltransferase Recognition Motif Identified in Chania multitudinisentens RB-25(T) gen. nov., sp. nov. Front Microbiol; 206, 31;7:1362. PubMedID: 27630623 , DOI: 10.3389/fmicb.2016.01362
Blow, M.J., Clark, T.A., Daum, C.G., Deutschbauer, A.M., Fomenkov, A., Fries, R., Froula, J., Kang, D.D., Malmstrom, R.R., Morgan, R.D., Posfai, J., Singh, K., Visel, A., Wetmore, K., Zhao, Z., Rubin, E.M., Korlach, J., Pennacchio, L.A. and Roberts, R.J. (2016) The Epigenomic Landscape of Prokaryotes. PLoS Genet; Feb 12;12(2):e1005854, PubMedID: 26870957, DOI: 10.1371/journal.pgen.1005854
Callahan, S.J., Luyten, Y.A., Gupta, Y.K., Wilson, G.G., Roberts, R.J., Morgan, R.D. and Aggarwal, A.K. (2016) Structure of Type IIL Restriction-Modification Enzyme MmeI in Complex with DNA Has Implications for Engineering New Specificities. PLoS Biol; Apr 15;14(4):e1002442, PubMedID: 27082731
Anton, B.P., Mongodin, E.F., Agrawal, S., Fomenkov, A., Byrd, D.R., Roberts, R.J. and Raleigh, E.A. (2015) Complete Genome Sequence of ER2796, a DNA Methyltransferase-Deficient Strain of Escherichia coli K-12 PLoS One; 10(5), e0127446. PubMedID: 26010885
Fomenkov, A., Lunnen, K.D., Zhu, Z., Anton, B.P., Wilson, G.G., Vincze, T. and Roberts, R.J. (2015) Complete genome sequence and methylome analysis of bacillus strain x1 Genome Announc; 3(1), PubMedID: 25700417
Xu, S-Y., Boitano, M., Clark, T.A., Vincze, T., Fomenkov, A., Kumar, S., Too, PH-M., Gonchar, D., Degtyarev, S.K. and Roberts, R.J. (2015) Complete Genome Sequence Analysis of Bacillus Subtilis T30 Genome Announc; 3(3), e00395-15. PubMedID: 25953183
Seib, K.L., Jen, F.E., Tan, A., Scott, A.L., Kumar, R., Power, P.M., Chen, L.T., Wu, H.J., Wang, A.H., Hill, D.M., Luyten, Y.A., Morgan, R.D., Roberts, R.J., Maiden, M.C., Boitano, M., Clark, T.A., Korlach, J., Rao, D.N. and Jennings, M.P. (2015) Specificity of the ModAll, ModA12 and ModD1 epigenetic regulator N6-adenine DNA methyltransferases of Neisseria Meningitidis Nucleic Acids Res; 43(8), 4150-4162. PubMedID: 25845594, DOI: 10.1093/nar/gkv219
Pirone-Davies, C., Hoffmann, M., Roberts, R.J., Muruvanda, T., Timme, R.E., Strain, E., Luo, Y., Payne, J., Luong, K., Song, Y., Tsai, Y.C., Boitano, M., Clark, T.A., Korlach, J., Evans, P.S., Allard, M.W. (2015) Genome-Wide Methylation Patterns in Salmonella enterica Subsp. enterica Serovars PLoS One; 10(4), e0123639. PubMedID: 25860355
Roberts, R.J., Vincze, T., Posfai, J., Macelis, D. (2014) REBASE - A database for DNA restriction and modification: enzymes, genes and genomes Nucleic Acids Res; PubMedID: 25378308
Bottacini, F., Morrissey, R., Roberts, R.J., James, J., van Breen, J., Egan, M., Lambert, J., van Limpt, K., Knol, J., O'Connell Motherway, M. and van Sinderen, D. (2018) Comparative genome and methylome analysis reveals restriction/modification system diversity in the gut commensal Bifidobacterium breve. Nucleic Acids Res; 46(4), 1860-1877. PubMedID: 29294107, DOI: 10.1093/nar/gkx1289
Deptula, P., Smolander, O.-P., Laine, P., Roberts, R.J., Edelmann, M., Peltola, P., Piironen, V., Paulin, L., Storgards, E., Savijoki, K., Laitila, A., Auvinen, P., Varmanen, P (2018) Acidipropionibacterium virtanenii sp nov isolated from malted barley Int J Syst Evol Microbiol; 68, 3175-3183. PubMedID: 30156530
Murray, Iain; Morgan, Rick; Luyten, Yvette; Fomenkov, Alexey; Correa, Ivan; Dai, Nan; Roberts, Rich; (2018) The non-specific adenine DNA methyltransferase M.EcoGII. Nucleic Acids Res; (2), 840-848. PubMedID: 29228259, DOI: 10.1093/nar/gkx1191
Anton, B.P., Roberts, R.J., Fomenkov, A., Humbert, A., Stoian, N., Zeilstra-Ryalls, J. (2018) Complete genome sequences of two Rhodobacter strains Microbiol Resour Announc; 7 (12), e01162-18. PubMedID: 30533667
Fomenkov, A., Vincze, T., Mersha, F., Roberts, R.J. (2018) Complete genome sequence and methylome analysis of Bacillus caldolyticus NEB414. Genome Announ.; 6 (6), e01605-17. PubMedID: 29439055 , DOI: 10.1128/genomeA.01605-17
Mauris, J.and Evans, T.C., Jr. (2010) A human PMS2 homologue from Aquifex aeolicus stimulates an ATP-dependent DNA helicase. J Biol Chem; 285(15), 11087-11092. PubMedID: 20129926

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