NEBNext^® Companion Module for Oxford Nanopore Technologies^® Ligation Sequencing

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
E7180S     -20       NEBNext® FFPE DNA Repair Buffer E7181AVIAL -20 1 x 0.084 ml Not Applicable   NEBNext® FFPE DNA Repair Mix E7182AVIAL -20 1 x 0.048 ml Not Applicable   NEBNext® Ultra™ II End Prep Reaction Buffer E7183AVIAL -20 1 x 0.084 ml Not Applicable   NEBNext® Ultra™ II End Prep Enzyme Mix E7184AVIAL -20 1 x 0.072 ml Not Applicable   Quick T4 DNA Ligase E7185AVIAL -20 1 x 0.24 ml Not Applicable
E7180L     -20       NEBNext® FFPE DNA Repair Buffer E7181AAVIAL -20 1 x 0.336 ml Not Applicable   NEBNext® FFPE DNA Repair Mix E7182AAVIAL -20 1 x 0.192 ml Not Applicable   NEBNext® Ultra™ II End Prep Reaction Buffer E7183AAVIAL -20 1 x 0.336 ml Not Applicable   NEBNext® Ultra™ II End Prep Enzyme Mix E7184AAVIAL -20 1 x 0.288 ml Not Applicable   Quick T4 DNA Ligase E7185AAVIAL -20 1 x 0.96 ml Not Applicable

Features

Component volumes tailored for use with singleplex SQK-LSK114 ligation sequencing

Simplified ordering and inventory management

Compatible with all devices: MinION^®, GridION^®, PromethION™, Flongle^®

No more waste – no unnecessary buffers or excess reagents

1. Usage guidelines for reagents included in the NEBNext^® Companion Module for Oxford Nanopore Technologies^® Ligation Sequencing (NEB #E7180)

1. Which protocol should be followed when using the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing?
2. What type and how much starting material do I need to use when preparing libraries using the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing?
3. Are the reagents supplied in the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing sufficient to construct libraries for Oxford Nanopore technologies devices?
4. Are the reagents supplied in the NEBNext^® Companion Module for Oxford Nanopore Technologies^® Ligation Sequencing recommended for native barcoding multiplex sequencing?
5. What is the difference between NEBNext^® Companion Module v2 for Oxford Nanopore Technologies^® Ligation Sequencing (NEB #E7672) & NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing (NEB #E7180)?
6. Can I still use the NEBNext^® Companion Module for Oxford Nanopore Technologies^® Ligation Sequencing (NEB #E7180) for SQK-LSK114 sequencing?

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Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• E7180S_v1
• E7180S_L_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• E7180S_v1_10044135
• E7180S_v1_10048498
• E7180S_v1_10050176
• E7180S_v1_10051829
• E7180S_v1_10053969
• E7180S_v1_10056179
• E7180S_v1_10057476
• E7180S_v1_10058069
• E7180S_v1_10059127
• E7180S_v0_10060365
• E7180S_v1_10062234
• E7180S_v1_10064509
• E7180S_v1_10057119
• E7180S_v1_10071820
• E7180S_v1_10072982
• E7180S_v1_10074406
• E7180S_v1_10077494
• E7180S_v1_10068792
• E7180S_v1_10086493
• E7180S_v1_10090199
• E7180S_v1_10080833
• E7180S_v1_10098362
• E7180S_v1_10100521
• E7180S_v1_10103026
• E7180S_v1_10104925
• E7180S_v1_10094146
• E7180S_v1_10110746
• E7180S_v1_10112718
• E7180S_v1_10115094
• E7180S_v1_10120344
• E7180S_v1_10108533
• E7180S_v1_10126631
• E7180S_v1_10127527
• E7180S_v1_10128600
• E7180S_v1_10122435
• E7180L_v2_10153481
• E7180S_v2_10153458
• E7180S_v2_10156263
• E7180S_v2_10148939
• E7180S_v2_10170552
• E7180S_v2_10172446
• E7180L_v2_10171818
• E7180S_v2_10164931
• E7180L_v2_10166928
• E7180S_v2_10179624
• E7180L_v2_10174744
• E7180S_v2_10174738
• E7180L_v2_10192912
• E7180S_v2_10192918
• E7180L_v2_10198493
• E7180S_v2_10202766
• E7180L_v2_10186364
• E7180S_v2_10186358
• E7180L_v2_10204116
• E7180S_v2_10204111
• E7180L_v2_10217687
• E7180S_v2_10217693
• E7180L_v2_10231267
• E7180S_v2_10231261
• E7180S_v2_10246255
• E7180L_v2_10246259

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• NEBNext^® FFPE DNA Repair Buffer
• NEBNext^® FFPE DNA Repair Mix
• NEBNext^® Ultra™ II End Prep Reaction Buffer
• NEBNext^® Ultra™ II End Prep Enzyme Mix
• Quick T4 DNA Ligase

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.