Genomic DNA Extraction & Purification

The ability to extract and purify gDNA from samples of interest is an essential first step in the study of many biological processes. Purification of gDNA often begins with homogenization of the sample followed by lysis of the cells to release the gDNA. RNase is typically employed to remove contaminated RNA from the lysate. The gDNA is then conditioned to selectively bind to a capture column, where it is subsequently washed to remove salts and other contaminants prior to elution.

NEB offers two approaches for the extraction and purification of gDNA: the Monarch Spin gDNA Purification Kit, which is a silica spin column-based process and the Monarch HMW DNA Extraction Kits, which utilize a novel glass bead-based process for purification of extremely high molecular weight DNA. The Monarch Spin gDNA Purification Kit is a universal kit for DNA extraction and purification from a wide variety of cell types, including blood, cells, tissues and tough-to-lyse samples (including bacteria and yeast).

The kit includes lysis buffers for various input materials, Proteinase K for homogenization of some sample types, and RNase A for efficient RNA removal. Genomic DNA (gDNA) is eluted with high yield and purity, and with excellent integrity (high molecular weight), ready for use in downstream applications including qPCR, long range PCR, and NGS library prep (including for long read sequencing platforms).

Choose Monarch for genomic DNA extraction and purification from a wide range of sample types

Purify high-quality, genomic DNA from a broad range of sample types (cells, blood, tissues, and more).