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High Molecular Weight DNA Extraction

TGlass plate with many Monarch® high molecular weight (HMW) glass beads on ithe Monarch® HMW DNA Extraction Kits provide a rapid and reliable process for extracting high molecular weight DNA (HMW DNA) from biological samples. NEB offers two approaches for the extraction and purification of gDNA: the Monarch Genomic DNA Purification Kit, which is a silica spin column-based process, and the Monarch HMW DNA Extraction Kits, which utilize a novel glass bead-based process for the purification of extremely high molecular weight DNA.

These kits utilize an optimized process that combines gentle cell lysis with a tunable fragment length generation, followed by precipitation of the extracted DNA onto the surface of large glass beads. Extracted DNA size ranges from 50-250 kb for the standard protocol and into the Mb range for certain sample types when the lowest agitation speeds are used.

Purified DNA is recovered in high yield with excellent purity, including nearly complete removal of RNA, and is ready for use in downstream applications, including long-read sequencing.

 

Workflow of high molecular weight gDNA extraction for blood, cells and tissue nucleic acid purification

 

Extract HMW DNA from cells and blood reproducibly with Monarch HMW DNA Extraction Kits

 

Reproducible Extraction of HMW DNA from Cells and Blood with the Monarch HMW DNA Extraction Kit

Gel photo showing cells blood reproducibility

DNA extracted with Monarch HMW DNA Extraction Kit for Cells & Blood. 1 x 106 fresh HEK293 cells and 500 µl fresh human blood were used as inputs and for preps performed according to the kit instructions using the agitation speed indicated above the gel lanes. 500 ng of DNA from the replicates was resolved by PFGE (1% agarose gel, 6 V/cm, 13°C for 20 hours, switch times ramped from 0.5–94 seconds on a BioRad® CHEF-DR® III System). Yield and purity ratios of the individual preps are shown in the accompanying tables. Lambda PFG Ladder (NEB #N0341) was used as molecular weight standard.

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High Molecular Weight DNA Extraction
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Monarch StabiLyse DNA/RNA Buffer

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Monarch® 2 ml Tubes

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Monarch® gDNA Elution Buffer II

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Monarch® gDNA Nuclei Prep & Lysis Buffer Pack

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Monarch® gDNA Wash Buffer

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Monarch® HMW DNA Extraction Kit for Cells & Blood

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Monarch® HMW DNA Extraction Kit for Tissue

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Monarch® HMW gDNA Tissue Lysis Buffer

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Monarch® Pestle Set

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Monarch® Precipitation Enhancer

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Monarch® Protein Separation Solution

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Monarch® RBC Lysis Buffer

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Monarch® RNase A

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Monarch® Spin Columns S2C and Tubes

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Monarch® DNA Capture Beads


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FAQs for High Molecular Weight DNA Extraction
Protocols for High Molecular Weight DNA Extraction
Application Notes for High Molecular Weight DNA Extraction
Legal Information

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. All other trademarks are the property of their respective owners. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

 


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