PCR & DNA Cleanup
Monarch Spin PCR & DNA Cleanup solutions reliably purify highly concentrated, high-quality double-stranded and single-stranded DNA from PCR, restriction digestions and reverse transcription. It utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns provided with each kit ensure zero buffer retention and no carryover of contaminants, enabling elution in volumes as low as 5 µl. Monarch Buffers have been optimized, and do not require pH monitoring. Eluted DNA is ready for use in restriction digests, DNA sequencing, PCR, ligation, and other enzymatic manipulations. Purify small DNA fragments, including oligonucleotides, with this single kit, elute in as little as 5 µl, and complete the entire workflow in only 5 minutes. The kit produces more concentrated DNA with better DNA recovery and purity compared to the leading supplier!
Purify oligonucleotide DNA with a dedicated oligo cleanup protocol with Monarch Spin PCR & DNA Cleanup
Choose Product:
Choose Type:
- Can the Monarch® Spin PCR & DNA Cleanup Kit (5 μg) (NEB #T1130) be used to purify RNA?
- Can the Monarch PCR & DNA Cleanup Kit (5 μg) be used to purify RNA?
- Can I purchase additional Monarch® columns and collection tubes for the MonarchSpin DNA Gel Extraction Kit (NEB #T1120) and the Monarch Spin PCR & DNA Cleanup Kit (NEB #T1130) separately?
- What is the smallest volume of elution buffer that can be used with the Monarch® Spin Columns S1A (NEB #T2037)?
- Do you have any recommendations for the purification of single-stranded DNA (ssDNA) when using the Monarch® Spin PCR & DNA Cleanup Kit (NEB #T1130)?
- Are the columns in the Monarch® Spin PCR & DNA Cleanup Kit (5 μg) (NEB #T1130) the same as those in the Monarch Spin DNA Gel Extraction Kit (NEB #T1120)?
- What size DNA can be purified with the Monarch® Spin Columns S1A (NEB #T2037)?
- Are Monarch® Spin Columns S1A (NEB #T2037) compatible with vacuum manifolds?
- What is the maximum binding capacity of the Monarch® Spin Columns S1A provided with the Monarch Spin PCR & DNA Cleanup Kit (5 μg) (NEB #T1130)?
- What factors affect the (A260/230) ratio when using the Monarch® Spin PCR & DNA Cleanup Kit (NEB #T1130)?
- What size primers can be effectively removed from a PCR reaction using the Monarch® Spin PCR & DNA Cleanup Kit (NEB #T1130)?
- Can I use the Monarch® Spin PCR & DNA Cleanup Kit (NEB #T1130) to purify oligonucleotides and other DNA fragments?
- Monarch DNA Purification Brochure
- Troubleshooting Guide for DNA Cleanup and Plasmid Purification
- Back to basics: Important things to keep in mind when purifying plasmids and DNA fragments
- Tips for the Monarch PCR & DNA Cleanup Kit
Brochures
Troubleshooting Guides
Usage Guidelines
- Elute in as low as 5 µl for highly concentrated DNA
- Prevent buffer retention and salt carry over with unique, optimized column design
- Reduce hands-on time with faster protocols and less spin timeNo need to monitor pH
- Protocol modification for oligonucleotide cleanup is provided, allowing purification of ssDNA, oligonucleotide and other small DNA fragments
- Significantly less plastic by design in columns and kit compared to leading suppliers
| Properties | |
|---|---|
| Purification format | Spin-column |
| DNA sample |
DNA from PCR and other enzymatic reactions (e.g., restriction digests, kinase reactions, ligations) |
| ssDNA or dsDNA oligonucleotides from enzymatic reactions can also be purified using the Oligonucleotide Cleanup Protocol | |
| Typical recovery |
70-90% |
| DNA Purity | A260/280 > 1.8 and A260/230 > 1.8 |
| Binding Capacity | Up to 5 μg |
| Elution Volume | 5-20 μl |
| DNA Size Range |
Standard protocol: 50 bp – 25 kb |
| Protocol Time | 5 minutes of spin and incubation time |
| Compatible Downstream Applications | Ligation, restriction digestion, labeling and other enzymatic manipulations, library construction and DNA sequencing |
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. All other trademarks are the property of their respective owners. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Learn about our
sustainability efforts
Explore our Monarch sustainability efforts.
Learn about our
unique column design
Monarch columns offer improved performance.
View more about our columns
See how Monarch compares
Monarch kits offer nucleic acid purifications solutions at the same or better quality than the leading supplier.
Ineligible item added to cart
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly. If you have any questions, please contact Customer Support at freezers@neb.com or 1-800-632-5227 x 8.
