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Plasmid Purification

Monarch nucleic acid purification kits provide fast and reliable purification of high quality DNA from bacterial cultures, agarose gels, and enzymatic reactions using best-in-class silica column technology.

The purification of plasmid DNA from bacterial cells is an important step in the cloning workflow. During plasmid purification, bacterial cells are lysed, freeing DNA and other cellular components from the cell wall. Cellular components are then removed, and the DNA-containing lysate is processed to further remove contaminants and separate the plasmid DNA from the genomic DNA.

Isolate high-quality, concentrated plasmid DNA with Monarch Kits

Purify up to 20 µg of plasmid DNA of various sizes, with elution in as little as 30 µl. Providing consistently high purity and quality concentrated DNA for use in downstream applications, such as restriction enzyme digestion, DNA sequencing, PCR, other enzymatic manipulations and transfection of robust cell lines.

Workflow of plasmid miniprep for nucleic acid purification

 

Monarch® Spin Plasmid Miniprep Kits provide a rapid and reliable method for the purification of high-quality plasmid DNA from bacterial cultures. The kit workflow employs standard cell resuspension, alkaline lysis, and neutralization steps, with the additional benefit of color indicators to easily monitor step completion.

Monarch kits for plasmid purification enable the purification of up to 20 µg of plasmid DNA from bacterial cultures. Buffers and columns are available separately for added convenience and all Monarch kits are designed with sustainability in mind — without compromising quality or value.

Isolate high-quality, concentrated plasmid DNA with Monarch Kits for Plasmid Purification

Purify up to 20 µg of plasmid DNA of various sizes, with elution in as little as 30 µl. Providing consistently high purity and quality concentrated DNA for use in downstream applications, such as restriction enzyme digestion, DNA sequencing, PCR, other enzymatic manipulations and transfection of robust cell lines.

Monarch Spin Plasmid Miniprep Kit consistently produces more concentrated plasmid DNA with equivalent or better yield and purity compared to the leading supplier

Graph showing concentrated DNA

DNA concentration, yield and purity are higher with Monarch Spin Plasmid Miniprep Kit than leading supplier’s kit, across different plasmids. Preps were performed according to recommended protocols using ~1 ml (OD600 = 3) aliquot for pUC19 and pHT01 and ~2 ml (OD600 = 6) aliquot for Rluc and pXba of the same overnight cultures. Concentrations of plasmid were measured using a Trinean DropSense 16.

 

Easily monitor protocol progression with an optimized buffer system included in Monarch kits for Plasmid Purification

Crop of Monarch® Spin Plasmid Miniprep Kit box with plastic bag of columns and bottles with colored buffers
Unique colored buffers prevent confusion and allow for monitoring of certain steps in the protocol. RNase is provided as part of the neutralization buffer, so there is no need to add it before starting — and no risk of forgetting to add it. Our specially formulated wash buffers ensure that salts, proteins, RNA and other cellular components are removed, allowing for the elution of concentrated, highly pure DNA.

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Plasmid Purification
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Monarch StabiLyse DNA/RNA Buffer

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Monarch® Buffer B1

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Monarch® Buffer B2

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Monarch® Buffer B3

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Monarch® Buffer BZ

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Monarch® Buffer WZ

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Monarch® Plasmid Miniprep Columns 

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Monarch® Spin Columns S2D and Tubes

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Monarch® Spin Plasmid Miniprep Kit


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FAQs for Plasmid Purification
Protocols for Plasmid Purification
Application Notes for Plasmid Purification
Legal Information

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. All other trademarks are the property of their respective owners. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

 


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Icon of bar graph and trend line representing performance data Monarch kits offer nucleic acid purifications solutions at the same or better quality than the leading supplier.

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