
Plasmid Purification
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- Can I purchase Monarch columns and collection tubes separately?
- Can I use water to elute the DNA when using the Monarch Kits?
- Are plasmids recovered using the Monarch® Plasmid Miniprep Kit endotoxin free?
- I have used 5 ml of cell culture for plasmid isolation with the Monarch Plasmid Miniprep kit and I am obtaining low amounts of plasmid and/or contaminating gDNA. Why is this, and what are your suggestions to improve yield and purity?
- For the Monarch® Spin Plasmid Miniprep Kit (NEB #T1110), which culture media do you recommended for growing E.coli?
- Which E.coli strain(s) are recommended for use with the Monarch® Spin Plasmid Miniprep Kit (NEB #T1110)?
- What culture conditions do you recommended for optimal performance with the Monarch® Spin Plasmid Miniprep Kit (NEB #T1110)?
- How can I prevent RNA contamination in DNA samples when using the Monarch® Spin Plasmid Miniprep Kit (NEB #T1110)?
- What factors affect my (A260/280) after performing a plasmid miniprep using the Monarch® Spin Plasmid Miniprep Kit (NEB #T1110)?
- What factors affect the recovery of plasmid DNA when using the Monarch® Spin Plasmid Miniprep Kit (NEB #T1110)?
- What factors affect my (A260/230) after performing a plasmid miniprep using the Monarch® Spin Plasmid Miniprep Kit (NEB #T1110)?
- What should I do differently when using a low copy plasmid?
- Do the dyes in the buffer of the Monarch® Spin Plasmid Miniprep Kit (NEB #T1110) affect downstream DNA applications?
- Can I use DNA isolated from the Monarch Plasmid Miniprep Kit for transfection?
- Are Monarch® Spin Columns S2D (NEB #T1117) compatible with vacuum manifolds?
- Troubleshooting Guide for DNA Cleanup and Plasmid Purification
- Back to basics: Important things to keep in mind when purifying plasmids and DNA fragments
- Dos and Don'ts of Plasmid Minipreps
- Usage Guidelines for the Monarch Plasmid Miniprep Kit (#T1010) When Working with Low Copy Plasmids
Troubleshooting Guides
Usage Guidelines
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