Total RNA Extraction & Purification

RNA plays a key role in gene expression and regulation. Isolation, extraction and purification of RNA from biological samples is an essential first step in the study of many biological processes. RNA extraction starts with homogenization of the samples followed by lysis of the cells to release the RNA. RNA is then conditioned to bind to a capture column and washed to remove salts and other contaminants before it is eluted. Any contaminating genomic DNA can be removed with column-based or enzyme-based methods.

The Monarch Spin RNA Isolation Kit is a universal kit for total RNA extraction and purification from a wide variety of sample types including blood, cells, tissues, and tough-to-lyse samples (including bacterial and yeast). The kit includes a protection reagent for sample storage and lysis, Proteinase K for homogenization of some sample types, as well as gDNA removal columns and DNase I for gDNA removal.

The Monarch Spin RNA Isolation Kit purifies RNA ranging in size from full length RNA to intact miRNAs. This kit can be used to clean up RNA after enzymatic reactions before use in downstream workflows, including cDNA synthesis, RT-PCR, RT-qPCR and RNA-seq.

Purify high-quality RNA from multiple sample types and low input amounts using the Monarch Spin RNA Isolation Kit

Monarch Spin RNA Isolation Kit (Mini) is a versatile solution for high-quality RNA extraction from a wide variety of sample types.

Purify total RNA of all sizes, including RNA <200 nts with the Monarch Spin RNA Isolation Kit

Monarch Spin RNA Isolation Kit (Mini) successfully purifies small RNAs below 200 nucleotides, enabling a more faithful representation of the total RNA pool compared to a leading supplier.