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Master Mixes

For added convenience, many PCR polymerases from NEB are available in a master mix format.

For added convenience, many PCR polymerases from NEB are available in a master mix format. Each master mix contains the polymerase, dNTPs, buffer and other components optimized for each particular reaction. Options include OneTaq®, Taq, Quick-Load® Taq (NEB #M0271), Multiplex PCR (NEB #M0284), LongAmp® Taq (NEB #M0287) and LongAmp Hot Start Taq (NEB #M0533) in various concentrations and optimized buffer compositions. Phusion™ and Q5™ master mixes bring higher fidelity to PCR reactions, while NEBNext® High-Fidelity 2X PCR Master Mix (NEB #M0541) is specifically optimized for amplification of next-generation sequencing libraries.

OneTaq®, Quick-Load®, LongAmp® and NEBNext® are registered trademarks of New England Biolabs, Inc.
Phusion™ was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific. This product is manufactured by New England Biolabs, Inc. under agreement with, and under the performance specifications of Thermo Fisher Scientific.
Q5™ is a trademark of New England Biolabs, Inc.


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Master Mixes
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Hot Start Taq 2X Master Mix

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LongAmp® Hot Start Taq 2X Master Mix

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LongAmp® Taq 2X Master Mix

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Multiplex PCR 5X Master Mix

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NEBNext® Q5® Hot Start HiFi PCR Master Mix

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OneTaq® 2X Master Mix with Standard Buffer

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OneTaq® Hot Start 2X Master Mix with GC Buffer

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OneTaq® Hot Start 2X Master Mix with Standard Buffer

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OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer

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OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer

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OneTaq® Quick-Load® 2X Master Mix with Standard Buffer

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Phusion High-Fidelity PCR Master Mix with GC Buffer

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Phusion High-Fidelity PCR Master Mix with HF Buffer

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Phusion Hot Start Flex 2X Master Mix

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Q5® High-Fidelity 2X Master Mix

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Q5® Hot Start High-Fidelity 2X Master Mix

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Q5® Blood Direct 2X Master Mix

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Quick-Load® Taq 2X Master Mix

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Taq 2X Master Mix

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Taq 5X Master Mix

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WarmStart® Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG)


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FAQs for Master Mixes
Protocols for Master Mixes
Application Notes for Master Mixes
DNA Polymerase Selection Chart
NEB offers a guidelines for choosing the correct DNA polymerase for your application by providing a list of specific properites.

Several factors govern which polymerase should be used in a given application, including:


Template/product specificity:
Is RNA or DNA involved? Is the 3´ terminus at a gap, nick or at the end of the template? 

Removal of existing nucleotides: Will the nucleotide(s) be removed from the existing polynucleotide chain as part of the protocol? If so, will they be removed from the 5´ or the 3´ end? 

Thermal stability: Does the polymerase need to survive incubation at high temperature or is heat inactivation desirable? 

Fidelity: Will subsequent sequence analysis or expression depend on the fidelity of the synthesized products?

PCR Selection Tool
Choose from one of the largest selections of polymerases for PCR applications from the leader in enzyme technology and bring unparalleled confidence to your experiments.
Legal Information

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. All other trademarks are the property of their respective owners. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.