OneTaq® DNA Polymerases
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- Protocol for One<em>Taq</em> 2X Master Mix with GC Buffer (M0483)
- Protocol for One<em>Taq</em> Hot Start Quick-Load 2X Master Mix with Standard Buffer (M0488)
- One<em>Taq</em>® Quick-Load® 2X Master Mix with GC Buffer (M0487)
- PCR Protocol for <em>Taq</em> DNA Polymerase
- Protocol for OneTaq Hot Start DNA Polymerase (M0481)
- Protocol for OneTaq® 2X Master Mix with Standard Buffer (M0482)
- Protocol for OneTaq Hot Start Quick-Load 2X Master Mix with GC Buffer
- Protocol for One<em>Taq</em> Hot Start 2X Master Mix with GC Buffer (M0485)
- Protocol for OneTaq Hot Start 2X Master Mix with Standard Buffer (M0484)
- Guidelines for PCR Optimization with OneTaq® and OneTaq® Hot Start DNA Polymerases
- Protocol for OneTaq® Quick-Load 2X Master Mix with Standard Buffer (M0486)
- PCR Protocol for OneTaq® DNA Polymerase (M0480)
- A-Tailing with Taq Polymerase
- Exceptional performance in endpoint PCR across a wide range of template
- Robust yields with minimal optimization
- Convenient product formats (stand-alone enzyme, master mixes, and Quick-Load® formats)
- Hot start version allows room temperature reaction setup and does not require a separate activation step
- Compatible with standard Taq protocols
- High sensitivity PCR
- High throughput PCR
- Routine PCR
- GC-rich PCR
- AT-rich PCR
- Primer extension
- Colony PCR
- Long PCR (up to ~6 kb genomic)
Template/product specificity: Is RNA or DNA involved? Is the 3´ terminus at a gap, nick or at the end of the template?
Removal of existing nucleotides: Will the nucleotide(s) be removed from the existing polynucleotide chain as part of the protocol? If so, will they be removed from the 5´ or the 3´ end?
Thermal stability: Does the polymerase need to survive incubation at high temperature or is heat inactivation desirable?
Fidelity: Will subsequent sequence analysis or expression depend on the fidelity of the synthesized products?
Reactions containing high GC human genomic DNA templates were set up at room temperature. PCR experiments included 30 cycles. Purity (A) and Yield (B) were calculated via microfluidic analysis from triplicate reactions. OneTaq polymerases were used with GC Buffer. Some OneTaq reactions also contained High GC Enhancer (striped bars). Competitor polymerases were cycled according to manufacturer's recommendations and included GC enhancers when supplied (striped bars).
Amplification of a selection of high GC human genomic DNA templates demonstrates OneTaq performance. PCR experiments included 30 amplification cycles. Purity (A) and Yield (B) were calculated via microfluidic analysis from triplicate reactions. Competitor polymerases were cycled according to manufacturer's recommendations.
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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