
RNA Modification
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- A Typical Deadenylation Reaction (M0331)
- Cap-0 synthesis using Vaccinia Capping Enzyme (NEB #M2080)
- End-labeling Protocol
- Non-radioactive Phosphorylation with T4 PNK or T4 PNK (3´ phosphatase minus)
- A Typical Tailing Reaction (M0337)
- Protocol for Dephosphorylation of 5´-ends of DNA using Antarctic Phosphatase (NEB #M0289)
- Radioactive Labeling with T4 PNK or T4 PNK (3´ phosphatase minus)
- 2´-O-Methylation of Capped RNA (M0366)
- One-Step Cap-1 mRNA synthesis with Vaccinia Capping Enzyme (VCE) and mRNA Cap 2´-O-methyltransferase (2´-O-me) (NEB #M0366, #M2080)
- Labeling Protocol (M2080)
- Protocol for Dephosphorylation of 5´-ends of DNA using rSAP (NEB #M0371)
- Poly(A) Tailing of RNA using E. coli Poly(A) Polymerase (NEB# M0276)
- Ligation of a DNA or RNA oligo to the 3’ end of long ssRNA using High Conc. T4 RNA Ligase 1 (NEB #M0437)
- Decapping eukaryotic mRNA with RppH (NEB #M0356)
- Protocol for Dephosphorylation of 5´-ends of DNA using Quick Dephosphorylation Kit (M0508)
- Enzymatic PCR Cleanup Protocol (NEB #M0371)
- RNA Ligase Selection Chart
- RNA Polymerase Selection Chart
- mRNA Decapping Selection Chart
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